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1: Plant Physiol. 2008 May;147(1):30-40. Epub 2008 Mar 19.
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Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei.

Zhang C, Barthelson RA, Lambert GM, Galbraith DW.

Department of Plant Sciences , The University of Arizona, Tucson, AZ 85721, USA.

We describe a simple and highly effective means for global identification of genes that are expressed within specific cell types within complex tissues. It involves transgenic expression of nuclear-targeted green fluorescent protein in a cell-type-specific manner. The fluorescent nuclei are then purified from homogenates by fluorescence-activated sorting, and the RNAs employed as targets for microarray hybridization. We demonstrate the validity of the approach through the identification of 12 genes that are selectively expressed in phloem.

Publication Types:
PMID: 18354040 [PubMed - indexed for MEDLINE]

PMCID: PMC2330299