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1: Science. 2007 Mar 2;315(5816):1270-4.
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Reconstitution of DNA segregation driven by assembly of a prokaryotic actin homolog.

Garner EC, Campbell CS, Weibel DB, Mullins RD.

Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.

Multiple unrelated polymer systems have evolved to partition DNA molecules between daughter cells at division. To better understand polymer-driven DNA segregation, we reconstituted the three-component segregation system of the R1 plasmid from purified components. We found that the ParR/parC complex can construct a simple bipolar spindle by binding the ends of ParM filaments, inhibiting dynamic instability, and acting as a ratchet permitting incorporation of new monomers and riding on the elongating filament ends. Under steady-state conditions, the dynamic instability of unattached ParM filaments provides the energy required to drive DNA segregation.

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PMID: 17332412 [PubMed - indexed for MEDLINE]