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1: Curr Opin Chem Biol. 2007 Apr;11(2):159-65. Epub 2007 Feb 1.
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Metallocenter assembly of the hydrogenase enzymes.

Leach MR, Zamble DB.

Department of Chemistry, University of Toronto, 80 St George St, Toronto, ON Canada, M5S 3H6.

The biosynthesis of the [NiFe]- and [FeFe]-hydrogenase enzymes requires the activities of multiple proteins that assemble the intricate metallocenters on the enzyme precursor proteins in an energy-dependent process. These accessory proteins include enzymes that synthesize the non-protein iron ligands as well as metallochaperones for the delivery of nickel to the [NiFe]-hydrogenase. Over the past few years many of these proteins have been examined in vitro. The biochemical properties, in the context of the earlier genetic studies, provide a basis for assigning function to the individual accessory proteins and mapping out the sequential steps of the metallocenter assembly pathways. This framework will serve as a foundation for detailed mechanistic analysis of these complex biomolecular factories.

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PMID: 17275396 [PubMed - indexed for MEDLINE]