Your browser version may not work well with NCBI's Web applications. More information here...
1: Science. 2007 Feb 2;315(5812):646-9.
Related Articles, Links
Click here to read Click here to read
The DEAD-box RNA helicase Dbp5 functions in translation termination.

Gross T, Siepmann A, Sturm D, Windgassen M, Scarcelli JJ, Seedorf M, Cole CN, Krebber H.

Institut für Molekularbiologie und Tumorforschung der Philipps-Universität Marburg, Emil-Mannkopff-Strasse 2, 35037 Marburg, Germany.

In eukaryotes, termination of messenger RNA (mRNA) translation is mediated by the release factors eRF1 and eRF3. Using Saccharomyces cerevisiae as a model organism, we have identified a member of the DEAD-box protein (DBP) family, the DEAD-box RNA helicase and mRNA export factor Dbp5, as a player in translation termination. Dbp5 interacts genetically with both release factors and the polyadenlyate-binding protein Pab1. A physical interaction was specifically detected with eRF1. Moreover, we show that the helicase activity of Dbp5 is required for efficient stop-codon recognition, and intact Dbp5 is essential for recruitment of eRF3 into termination complexes. Therefore, Dbp5 controls the eRF3-eRF1 interaction and thus eRF3-mediated downstream events.

Publication Types:
PMID: 17272721 [PubMed - indexed for MEDLINE]