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1: Mol Cell. 2006 Dec 28;24(6):917-29.
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A genome-wide analysis indicates that yeast pre-mRNA splicing is predominantly posttranscriptional.

Tardiff DF, Lacadie SA, Rosbash M.

Biology Department MS008, Howard Hughes Medical Institute, Brandeis University, 415 South Street, Waltham, Massachusetts 02454, USA.

Recent ChIP experiments indicate that spliceosome assembly and splicing can occur cotranscriptionally in S. cerevisiae. However, only a few genes have been examined, and all have long second exons. To extend these studies, we analyzed intron-containing genes with different second exon lengths by using ChIP as well as whole-genome tiling arrays (ChIP-CHIP). The data indicate that U1 snRNP recruitment is independent of exon length. Recursive splicing constructs, which uncouple U1 recruitment from transcription, suggest that cotranscriptional U1 recruitment contributes to optimal splicing efficiency. In contrast, U2 snRNP recruitment, as well as cotranscriptional splicing, is deficient on short second exon genes. We estimate that > or =90% of endogenous yeast splicing is posttranscriptional, consistent with an analysis of posttranscriptional snRNP-associated pre-mRNA.

Publication Types:
PMID: 17189193 [PubMed - indexed for MEDLINE]

PMCID: PMC1828117