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1: PLoS Genet. 2006 Jun;2(6):e91. Epub 2006 Jun 9.
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Genetic screens for mutations affecting development of Xenopus tropicalis.

Goda T, Abu-Daya A, Carruthers S, Clark MD, Stemple DL, Zimmerman LB.

Division of Developmental Biology, National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom.

We present here the results of forward and reverse genetic screens for chemically-induced mutations in Xenopus tropicalis. In our forward genetic screen, we have uncovered 77 candidate phenotypes in diverse organogenesis and differentiation processes. Using a gynogenetic screen design, which minimizes time and husbandry space expenditures, we find that if a phenotype is detected in the gynogenetic F2 of a given F1 female twice, it is highly likely to be a heritable abnormality (29/29 cases). We have also demonstrated the feasibility of reverse genetic approaches for obtaining carriers of mutations in specific genes, and have directly determined an induced mutation rate by sequencing specific exons from a mutagenized population. The Xenopus system, with its well-understood embryology, fate map, and gain-of-function approaches, can now be coupled with efficient loss-of-function genetic strategies for vertebrate functional genomics and developmental genetics.

Publication Types:
PMID: 16789825 [PubMed - indexed for MEDLINE]

PMCID: PMC1475704