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1: Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2243-8. Epub 2006 Feb 6.
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SAD-2 is required for meiotic silencing by unpaired DNA and perinuclear localization of SAD-1 RNA-directed RNA polymerase.

Shiu PK, Zickler D, Raju NB, Ruprich-Robert G, Metzenberg RL.

Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA. shuip@missouri.edu

A gene unpaired during the meiotic homolog pairing stage in Neurospora generates a sequence-specific signal that silences the expression of all copies of that gene. This process is called Meiotic Silencing by Unpaired DNA (MSUD). Previously, we have shown that SAD-1, an RNA-directed RNA polymerase (RdRP), is required for MSUD. We isolated a second gene involved in this process, sad-2. Mutated Sad-2 (RIP) alleles, like those of Sad-1, are dominant and suppress MSUD. Crosses homozygous for Sad-2 are blocked at meiotic prophase. SAD-2 colocalizes with SAD-1 in the perinuclear region, where small interfering RNAs have been shown to reside in mammalian cells. A functional sad-2(+) gene is necessary for SAD-1 localization, but the converse is not true. The data suggest that SAD-2 may function to recruit SAD-1 to the perinuclear region, and that the proper localization of SAD-1 is important for its activity.

Publication Types:
PMID: 16461906 [PubMed - indexed for MEDLINE]

PMCID: PMC1413707