Your browser version may not work well with NCBI's Web applications. More information here...
1: Curr Microbiol. 2006 Jan;52(1):69-73. Epub 2005 Dec 26.
Related Articles, Links
Click here to read Click here to read
Green fluorescent protein labeling Escherichia coli TG1 confirms intestinal bacterial translocation in a rat model of chemotherapy.

Song D, Shi B, Xue H, Li Y, Yu B, Xu Z, Liu F, Li J.

Medical College, Nanjing University, Nanjing 210093, People's Republic of China.

It has been reported that treatment with methotrexate (MTX) induces intestinal bacterial translocation; however, the definitive evidence of intestinal bacterial translocation induced by MTX has been lacking. The aim of this study was to confirm the intestinal bacterial translocation caused by MTX and to evaluate the preventive effect of granulocyte colony-stimulating factor (G-CSF) on intestinal bacterial translocation caused by MTX. Sprague-Dawley rats were treated with MTX (3.5 mg/kg) for 3 days to induce intestinal bacterial translocation; with gavaged Escherichia coli TG1 labeled with green fluorescent protein (GFP) for 2 days to track intestinal bacterial translocation; and with G-CSF (10 mug/kg) for 4 days to prevent intestinal bacterial translocation. Representative tissue specimens from the mesenteric lymph nodes, spleen, liver, and kidney were aseptically harvested for bacteria culture in ampicillin-supplemented medium. The bacteria labeled with GFP were detected in tissue specimens harvested from the rats treated with MTX but not detected in the rats that were not treated with MTX. G-CSF significantly ameliorated the situation of intestinal bacterial translocation.

Publication Types:
PMID: 16392000 [PubMed - indexed for MEDLINE]