Your browser version may not work well with NCBI's Web applications. More information here...
1: Nat Immunol. 2005 Aug;6(8):836-43. Epub 2005 Jul 17.
Related Articles, Links
Click here to read Click here to read
Regulation of interleukin 7-dependent immunoglobulin heavy-chain variable gene rearrangements by transcription factor STAT5.

Bertolino E, Reddy K, Medina KL, Parganas E, Ihle J, Singh H.

Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois 60637, USA.

Rearrangement of immunoglobulin heavy-chain variable (V(H)) gene segments has been suggested to be regulated by interleukin 7 signaling in pro-B cells. However, the genetic evidence for this recombination pathway has been challenged. Furthermore, no molecular components that directly control V(H) gene rearrangement have been elucidated. Using mice deficient in the interleukin 7-activated transcription factor STAT5, we demonstrate here that STAT5 regulated germline transcription, histone acetylation and DNA recombination of distal V(H) gene segments. STAT5 associated with V(H) gene segments in vivo and was recruited as a coactivator with the transcription factor Oct-1. STAT5 did not affect the nuclear repositioning or compaction of the immunoglobulin heavy-chain locus. Therefore, STAT5 functions at a distinct step in regulating distal V(H) recombination in relation to the transcription factor Pax5 and histone methyltransferase Ezh2.

PMID: 16025120 [PubMed - indexed for MEDLINE]