Your browser version may not work well with NCBI's Web applications. More information here...
1: Nat Methods. 2004 Oct;1(1):47-53. Epub 2004 Sep 29.
Related Articles, Links
Click here to read Click here to read
Comment in:
A custom microarray platform for analysis of microRNA gene expression.

Thomson JM, Parker J, Perou CM, Hammond SM.

Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA.

MicroRNAs are short, noncoding RNA transcripts that post-transcriptionally regulate gene expression. Several hundred microRNA genes have been identified in Caenorhabditis elegans, Drosophila, plants and mammals. MicroRNAs have been linked to developmental processes in C. elegans, plants and humans and to cell growth and apoptosis in Drosophila. A major impediment in the study of microRNA function is the lack of quantitative expression profiling methods. To close this technological gap, we have designed dual-channel microarrays that monitor expression levels of 124 mammalian microRNAs. Using these tools, we observed distinct patterns of expression among adult mouse tissues and embryonic stem cells. Expression profiles of staged embryos demonstrate temporal regulation of a large class of microRNAs, including members of the let-7 family. This microarray technology enables comprehensive investigation of microRNA expression, and furthers our understanding of this class of recently discovered noncoding RNAs.

Publication Types:
PMID: 15782152 [PubMed - indexed for MEDLINE]