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1: Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8337-41. Epub 2004 May 17.
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Predicting indirect readout effects in protein-DNA interactions.

Zhang Y, Xi Z, Hegde RS, Shakked Z, Crothers DM.

Departments of Chemistry and Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511, USA.

Recognition of DNA by proteins relies on direct interactions with specific DNA-functional groups, along with indirect effects that reflect variable energetics in the response of DNA sequences to twisting and bending distortions induced by proteins. Predicting indirect readout requires knowledge of the variations in DNA curvature and flexibility in the affected region, which we have determined for a series of DNA-binding sites for the E2 regulatory protein by using the cyclization kinetics method. We examined 16 sites containing different noncontacted spacer sequences, which vary by more than three orders of magnitude in binding affinity. For 15 of these sites, the variation in affinity was predicted within a factor of 3, by using experimental curvature and flexibility values and a statistical mechanical theory. The sole exception was traced to differential magnesium ion binding.

Publication Types:
PMID: 15148366 [PubMed - indexed for MEDLINE]

PMCID: PMC420395