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1: Science. 2002 Jun 7;296(5574):1869-73. Epub 2002 May 16.
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Two-photon imaging of lymphocyte motility and antigen response in intact lymph node.

Miller MJ, Wei SH, Parker I, Cahalan MD.

Department of Physiology and Biophysics, University of California, Irvine, CA 92697-4561, USA.

Lymphocyte motility is vital for trafficking within lymphoid organs and for initiating contact with antigen-presenting cells. Visualization of these processes has previously been limited to in vitro systems. We describe the use of two-photon laser microscopy to image the dynamic behavior of individual living lymphocytes deep within intact lymph nodes. In their native environment, T cells achieved peak velocities of more than 25 micrometers per minute, displaying a motility coefficient that is five to six times that of B cells. Antigenic challenge changed T cell trajectories from random walks to "swarms" and stable clusters. Real-time two-photon imaging reveals lymphocyte behaviors that are fundamental to the initiation of the immune response.

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PMID: 12016203 [PubMed - indexed for MEDLINE]