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Karen Allen
Department of Physiology and Biophysics, Boston University School of Medicine, Boston, United States of America

Faculty Member: CHEMICAL BIOLOGY > Biocatalysis [ since 30 July 2003 ]
[ Biography ] [ Homepage ] [ Evaluations ]
Biography

Contact Details
Karen N. Allen
Department of Physiology and Biophysics
Boston University School of Medicine
715 Albany Street
Boston, MA 02118-2394
Tel: 617-638-4398
Fax: 617-638-4285
Email: drkallen@bu.edu

Associate Professor, Department of Physiology and Biophysics, Boston University School of Medicine. B.S. in Biology, 1984, Tufts University, cum laude, Ph.D. in Biochemistry, 1989, Brandeis University, supported as a Dretzin Scholar in the laboratory of Dr. Robert Abeles. American Cancer Society Postdoctoral Fellow, 1989-1993 in the laboratory of Drs. Gregory Petsko and Dagmar Ringe at MIT and Brandeis University, Chair, Gordon Research Conference on Enzymes Coenzymes and Metabolic Pathways (2002); Member, NIH Biochemistry Study Section (2002-present); Member NSF Molecular and Cellular Biosciences Panel (1997-2000); Member, Nominating Committee, American Chemical Society, Division of Biological Chemistry (2002)

Research Interests: Elucidation of enzyme mechanisms through X-ray crystallography and kinetics. Mechanism of phosphate transfer reactions, evolution of substrate specificity in enzyme superfamilies, deciphering moonlighting functions of enzymes via examination of protein-protein interactions on the molecular level.

Home page

http://biophysics.bumc.bu.edu/faculty/allen/

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Evaluations

Evaluation of the substrate envelope hypothesis for inhibitors of HIV-1 protease.
Chellappan S, Kairys V, …, Schiffer C, Gilson MK
Proteins 2007 Aug 1 68(2):561-7 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Karen Allen
Evaluated 19 Feb 2008

Structure-based activity prediction for an enzyme of unknown function.
Hermann JC, Marti-Arbona R, …, Shoichet BK, Raushel FM
Nature 2007 Aug 16 448(7155):775-9 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Rowena Matthews / Michael Gelb / Karen Allen / Reinhard Sterner / Andrea Mattevi / Giulio Superti-Furga / Shelley Copley
Evaluated 10 Aug 2007

Prediction and assignment of function for a divergent N-succinyl amino acid racemase.
Song L, Kalyanaraman C, …, Jacobson MP, Gerlt JA
Nat Chem Biol 2007 Aug 3(8):486-91 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Karen Allen
Evaluated 10 Aug 2007

Live-cell imaging of enzyme-substrate interaction reveals spatial regulation of PTP1B.
Yudushkin IA, Schleifenbaum A, …, Schultz C, Bastiaens PI
Science 2007 Jan 5 315(5808):115-9 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Kermit Carraway / Amy Barrios / Dafna Bar-Sagi / Karen Allen
Evaluated 23 May 2007

Redefining the requisite lipopolysaccharide structure in Escherichia coli.
Meredith TC, Aggarwal P, …, Lindner B, Woodard RW
ACS Chem Biol 2006 Feb 17 1(1):33-42 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Miguel Valvano / Karen Allen
Evaluated 1 Feb 2007

A new paradigm for DNA polymerase specificity.
Tsai YC, Johnson KA
Biochemistry 2006 Aug 15 45(32):9675-87 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Karen Allen / Arieh Warshel
Evaluated 12 Jan 2007

A survey of protein-protein complex crystallizations.
Radaev S, Li S, Sun PD
Acta Crystallogr D Biol Crystallogr 2006 Jun 62(Pt 6):605-12 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Karen Allen / Mark Hogarth
Evaluated 14 Aug 2006

Phosphate analogues as probes of the catalytic mechanisms of MurA and AroA, two carboxyvinyl transferases.
Zhang F, Berti PJ
Biochemistry 2006 May 16 45(19):6027-37 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Karen Allen
Evaluated 25 May 2006

A fluoroacetamidine-based inactivator of protein arginine deiminase 4: design, synthesis, and in vitro and in vivo evaluation.
Luo Y, Knuckley B, …, Stallcup MR, Thompson PR
J Am Chem Soc 2006 Feb 1 128(4):1092-3 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Karen Allen
Evaluated 13 Mar 2006

Activation of orotidine 5'-monophosphate decarboxylase by phosphite dianion: the whole substrate is the sum of two parts.
Amyes TL, Richard JP, Tait JJ
J Am Chem Soc 2005 Nov 16 127(45):15708-9 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Karen Allen / Arieh Warshel
Evaluated 13 Mar 2006

Is Arg418 the catalytic base required for the hydrolysis step of the IMP dehydrogenase reaction?
Guillén Schlippe YV, Hedstrom L
Biochemistry 2005 Sep 6 44(35):11700-7 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Karen Allen
Evaluated 6 Dec 2005

Intermediates trapped during nitrogenase reduction of N triple bond N, CH3-N=NH, and H2N-NH2.
Barney BM, Yang TC, …, Dean DR, Seefeldt LC
J Am Chem Soc 2005 Nov 2 127(43):14960-1 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Edward Stiefel / Jon Zubieta / Karen Allen / Joan Broderick
Evaluated 1 Dec 2005

Tryptophan 7-halogenase (PrnA) structure suggests a mechanism for regioselective chlorination.
Dong C, Flecks S, …, van Pée KH, Naismith JH
Science 2005 Sep 30 309(5744):2216-9 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Jon Thorson / Karen Allen / Mark Nelson
Evaluated 1 Dec 2005

An efficient one-step site-directed and site-saturation mutagenesis protocol.
Zheng L, Baumann U, Reymond JL
Nucleic Acids Res 2004 32(14):e115 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Karen Allen
Evaluated 4 Aug 2005

A fluoroquinolone resistance protein from Mycobacterium tuberculosis that mimics DNA.
Hegde SS, Vetting MW, …, Takiff HE, Blanchard JS
Science 2005 Jun 3 308(5727):1480-3 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Laurence Pearl / Torsten Schwede / Malcolm White / Xiayang Qiu / Brigitte Gicquel / Matt Redinbo / Peter Artymiuk / Mario Amzel / Karen Allen
Evaluated 25 Jul 2005

Phospholipids as determinants of membrane protein topology. Phosphatidylethanolamine is required for the proper topological organization of the gamma-aminobutyric acid permease (GabP) of Escherichia coli.
Zhang W, Campbell HA, King SC, Dowhan W
J Biol Chem 2005 Jul 15 280(28):26032-8 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Karen Allen
Evaluated 25 Jul 2005

General acid catalysis by the hepatitis delta virus ribozyme.
Das SR, Piccirilli JA
Nat Chem Biol 2005 Jun 1(1):45-52 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Karen Allen / David M.J. Lilley / Daniel Herschlag
Evaluated 3 Jun 2005

Femtomolar transition state analogue inhibitors of 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase from Escherichia coli.
Singh V, Evans GB, …, Howell PL, Schramm VL
J Biol Chem 2005 May 6 280(18):18265-73 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Karen Allen
Evaluated 1 Apr 2005

Mechanism of the Schiff base forming fructose-1,6-bisphosphate aldolase: structural analysis of reaction intermediates.
Lorentzen E, Siebers B, Hensel R, Pohl E
Biochemistry 2005 Mar 22 44(11):4222-9 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Karen Allen
Evaluated 24 Mar 2005

Closing the folding chamber of the eukaryotic chaperonin requires the transition state of ATP hydrolysis.
Meyer AS, Gillespie JR, …, Doniach S, Frydman J
Cell 2003 May 2 113(3):369-81 [abstract on PubMed] [related articles] [order article]
Selected by | Laurence Pearl / Ulrich Hartl / Karen Allen
Evaluated 18 Aug 2004

The perfect penicillin? Inhibition of a bacterial DD-peptidase by peptidoglycan-mimetic beta-lactams.
Josephine HR, Kumar I, Pratt RF
J Am Chem Soc 2004 Jul 7 126(26):8122-3 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Karen Allen
Evaluated 11 Aug 2004

Occurrence of a putative ancient-like isomerase involved in histidine and tryptophan biosynthesis.
Barona-Gómez F, Hodgson DA
EMBO Rep 2003 Mar 4(3):296-300 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Karen Allen
Evaluated 17 Mar 2004

Versatile fluorescence probes of protein kinase activity.
Shults MD, Imperiali B
J Am Chem Soc 2003 Nov 26 125(47):14248-9 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Karen Allen
Evaluated 8 Mar 2004

Metaphosphate in the active site of fructose-1,6-bisphosphatase.
Choe JY, Iancu CV, Fromm HJ, Honzatko RB
J Biol Chem 2003 May 2 278(18):16015-20 [abstract on PubMed] [related articles] [FREE full text]
Selected by | Karen Allen / Arieh Warshel
Evaluated 3 Feb 2004

Predictive crystallization of ribonuclease A via rapid screening of osmotic second virial coefficients.
Tessier PM, Johnson HR, …, Sandler SI, Lenhoff AM
Proteins 2003 Feb 1 50(2):303-11 [abstract on PubMed] [related articles] [full text] [order article]
Selected by | Karen Allen
Evaluated 7 Oct 2003

Aldolase forms a bridge between cell surface adhesins and the actin cytoskeleton in apicomplexan parasites.
Jewett TJ, Sibley LD
Mol Cell 2003 Apr 11(4):885-94 [abstract on PubMed] [related articles] [order article]
Selected by | Karen Allen
Evaluated 29 Aug 2003

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